Phenotype microarray analysis may unravel genetic determinants of the stress response by Rhodococcus aetherivorans BCP1 and Rhodococcus opacus R7

Martina Cappelletti; Stefani Fedi; Jessica Zampolli; Alessandra Di Canito; Pasqualina D'Ursi; Alessandro Orro; Carlo Viti; Luciano Milanesi; Davide Zannoni; Patrizia Di Gennaro

doi:10.1016/j.resmic.2016.06.008

Phenotype microarray analysis may unravel genetic determinants of the stress response by Rhodococcus aetherivorans BCP1 and Rhodococcus opacus R7

Res Microbiol. 2016 Nov-Dec;167(9-10):766-773. doi: 10.1016/j.resmic.2016.06.008. Epub 2016 Jul 7.

Authors

Affiliations

¹ Department of Pharmacy and Biotechnology, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy. Electronic address: martina.cappelletti2@unibo.it.
² Department of Pharmacy and Biotechnology, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy. Electronic address: stefano.fedi@unibo.it.
³ Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy. Electronic address: j.zampolli@campus.unimib.it.
⁴ Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy. Electronic address: a.dicanito@campus.unimib.it.
⁵ CNR, Institute for Biomedical Technologies, Via Fratelli Cervi 93, 20090 Segrate, Milan, Italy. Electronic address: pasqualina.dursi@itb.cnr.it.
⁶ CNR, Institute for Biomedical Technologies, Via Fratelli Cervi 93, 20090 Segrate, Milan, Italy. Electronic address: alessandro.orro@itb.cnr.it.
⁷ Department of Agrifood Production and Environmental Sciences, University of Firenze, Piazzale delle Cascine 24, 50144 Florence, Italy. Electronic address: carlo.viti@unifi.it.
⁸ CNR, Institute for Biomedical Technologies, Via Fratelli Cervi 93, 20090 Segrate, Milan, Italy. Electronic address: luciano.milanesi@gmail.com.
⁹ Department of Pharmacy and Biotechnology, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy. Electronic address: davide.zannoni@unibo.it.
¹⁰ Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy. Electronic address: patrizia.digennaro@unimib.it.

PMID: 27394988
DOI: 10.1016/j.resmic.2016.06.008

Abstract

In the present study, the response of Rhodococcus aetherivorans BCP1 and Rhodococcus opacus R7 to various stress conditions and several antimicrobials was examined by PM in relation with genetic determinants, as revealed by annotation analysis of the two genomes. Comparison between metabolic activities and genetic features of BCP1 and R7 provided new insight into the environmental persistence of these two members of the genus Rhodococcus.

Keywords: Phenotype microarray; Rhodococcus; Rhodococcus aetherivorans BCP1; Rhodococcus opacus R7; Stress response; Toxic compounds.

MeSH terms

Anti-Bacterial Agents / metabolism
Genes, Bacterial
Genotype
Microarray Analysis*
Phenotype*
Rhodococcus / drug effects
Rhodococcus / genetics
Rhodococcus / physiology*
Stress, Physiological*

Substances

Anti-Bacterial Agents