[Rapid detection of antimicrobial resistance by MALDI-TOF mass spectrometry]

Enferm Infecc Microbiol Clin. 2016 Jun:34 Suppl 2:36-41. doi: 10.1016/S0213-005X(16)30189-6.
[Article in Spanish]

Abstract

In recent years, MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometry has become established as a first-line diagnostic tool in the identification of microorganisms, including those producing human infections. Rapid detection of antimicrobial resistance is one of the future applications of this technique with the greatest likelihood of success. This review describes the most important studies published in this field and discusses potential future challenges and the clinical application of this technique in the next few years.

Keywords: Detección rápida; Espectrometría de masas; MALDI-TOF; Mass spectrometry; Mecanismos de resistencia; Rapid detection; Resistance mechanisms.

Publication types

  • Review

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacteria / drug effects
  • Bacteria / enzymology
  • Bacterial Proteins / analysis
  • Drug Resistance, Bacterial*
  • Drug Resistance, Fungal*
  • Forecasting
  • Fungal Proteins / analysis
  • Humans
  • Isotope Labeling
  • Microbial Sensitivity Tests / instrumentation
  • Microbial Sensitivity Tests / methods*
  • Sequence Analysis, DNA / methods
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Yeasts / drug effects
  • Yeasts / enzymology

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Fungal Proteins