Chlamydiae are obligate intracellular bacteria with two distinct morphological stages, the infectious elementary bodies (EBs) and non-infectious reticulate bodies (RBs). Here we describe a rapid and straightforward protocol for the purification of EBs and RBs involving special density gradients. It has been successfully applied to three chlamydial species.
Keywords: Intracellular pathogens; Iodixanol; Optiprep™; Preparative ultracentrifugation; Visipaque®.
Copyright © 2016. Published by Elsevier B.V.