Purification of infectious and non-infectious chlamydial particles using iodixanol for density gradient preparation

J Microbiol Methods. 2016 Sep:128:20-23. doi: 10.1016/j.mimet.2016.06.029. Epub 2016 Jul 1.

Abstract

Chlamydiae are obligate intracellular bacteria with two distinct morphological stages, the infectious elementary bodies (EBs) and non-infectious reticulate bodies (RBs). Here we describe a rapid and straightforward protocol for the purification of EBs and RBs involving special density gradients. It has been successfully applied to three chlamydial species.

Keywords: Intracellular pathogens; Iodixanol; Optiprep™; Preparative ultracentrifugation; Visipaque®.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Chlamydia / classification
  • Chlamydia / isolation & purification*
  • Colony Count, Microbial
  • Contrast Media / chemistry*
  • Humans
  • RNA, Bacterial / isolation & purification*
  • RNA, Ribosomal, 16S / isolation & purification
  • Triiodobenzoic Acids / chemistry*

Substances

  • Contrast Media
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Triiodobenzoic Acids
  • iodixanol