Data set for diet specific differential gene expression analysis in three Spodoptera moths

A Roy; W B Walker 3rd; H Vogel; S K Kushwaha; S Chattington; M C Larsson; P Anderson; D G Heckel; F Schlyter

doi:10.1016/j.dib.2016.04.029

Data set for diet specific differential gene expression analysis in three Spodoptera moths

Data Brief. 2016 May 20:8:448-55. doi: 10.1016/j.dib.2016.04.029. eCollection 2016 Sep.

Authors

A Roy¹, W B Walker 3rd², H Vogel³, S K Kushwaha⁴, S Chattington¹, M C Larsson¹, P Anderson¹, D G Heckel³, F Schlyter¹

Affiliations

¹ Chemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, Sweden.
² Chemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, Sweden; Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745 Jena, Germany.
³ Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745 Jena, Germany.
⁴ Department of Plant Breeding, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, Sweden.

Abstract

Examination of closely related species pairs is suggested for evolutionary comparisons of different degrees of polyphagy, which we did here with three taxa of lepidopteran herbivores, Spodoptera spp (S. littoralis, S. frugiperda maize (C) and rice (R) strains) for a RNAseq analysis of the midguts from the 3rd instar insect larvae for differential metabolic responses after feeding on pinto bean based artificial diet vs maize leaves. Paired-end (2×100 bp) Illumina HiSeq2500 sequencing resulted in a total of 24, 23, 24, and 21 million reads for the SF-C-Maize, SF-C-Pinto, SF-R-Maize, SF-R Pinto, and a total of 35 and 36 million reads for the SL-Maize and SL-Pinto samples, respectively. After quality control measures, a total of 62.2 million reads from SL and 71.7 million reads from SF were used for transcriptome assembly (TA). The resulting final de novo reference TA (backbone) for the SF taxa contained 37,985 contigs with a N50 contig size of 1030 bp and a maximum contig length of 17,093 bp, while for SL, 28,329 contigs were generated with a N50 contig size of 1980 bp and a maximum contig length of 18,267 bp. The data presented herein contains supporting information related to our research article Roy et al. (2016) http://dx.doi.org/10.1016/j.ibmb.2016.02.006[1].

Keywords: Adaptation; Differential expression analysis (DGE); Generalist; RNA seq; RPKM (reads per kilo base of transcript per million mapped reads); Specialist; Spodoptera; Transcriptomics.