Investigation the efficiency of various methods of volatile oil extraction from Trichodesma africanum and their impact on the antioxidant and antimicrobial activities

Nidal Amin Jaradat; Abdel Naser Zaid; Aladdin Abuzant; Ramzi Shawahna

doi:10.5455/jice.20160421065949

Investigation the efficiency of various methods of volatile oil extraction from Trichodesma africanum and their impact on the antioxidant and antimicrobial activities

J Intercult Ethnopharmacol. 2016 May 4;5(3):250-6. doi: 10.5455/jice.20160421065949. eCollection 2016 Jun-Aug.

Authors

Nidal Amin Jaradat¹, Abdel Naser Zaid², Aladdin Abuzant¹, Ramzi Shawahna³

Affiliations

¹ Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, State of Palestine.
² Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, State of Palestine.
³ Department of Physiology, Pharmacology and Toxicology, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, State of Palestine.

Abstract

Background: Currently, there is an increasing interest in developing more efficient techniques for the extraction of phytochemicals. Microwaves and ultrasonic extraction methods are promising techniques that can be used for this purpose.

Objectives: The purpose of this study was to investigate the impact of different extraction methods on yield, antioxidant and antimicrobial activities of volatile oil extracted from Trichodesma africanum.

Materials and methods: Volatile oil was extracted using microwave, ultrasonic, microwave-ultrasonic, and conventional hydrodistillation methods. The extracted oil was evaluated for antioxidant and antimicrobial activities. The antioxidant activity was assessed by 2,2-Diphenyl-1-picrylhydrazyl scavenging assay, whereas the antimicrobial activity was assessed by broth microdilution method. The antimicrobial activity of the volatile oils was examined against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa American type culture collection reference strains, as well as against methicillin-resistant S. aureus (MRSA) and Candida albicans clinical isolates.

Results: The volatile oil obtained by the four extraction methods in this study exhibited both antioxidant and antimicrobial activities. Among the four extraction methods used, the microwave-ultrasonic method yielded the largest amount (1.8% v/w) and the yield exhibited the highest antioxidant activity in terms of inhibition (91.83% ± 1.1). The minimum inhibitory concentrations for E. coli, P. aeruginosa, S. aureus, MRSA, and C. albicans were 3, 5, 6, 3, and 9, respectively.

Conclusion: Among the extraction techniques used in this study, the microwave-ultrasonic method showed the best results. Moreover, this study suggests that T. africanum volatile oils contain active substances that could potentially be used both as natural preservatives in food and pharmaceutical industries as well as in developing new antimicrobial and antioxidant agents.

Keywords: Antimicrobial; Trichodesma africanum; antioxidant; microwave-ultrasonic.