Dihydroceramide-desaturase-1-mediated caspase 9 activation through ceramide plays a pivotal role in palmitic acid-induced HepG2 cell apoptosis

Apoptosis. 2016 Sep;21(9):1033-44. doi: 10.1007/s10495-016-1267-9.

Abstract

In this study, results showed that the inhibition of PA-induced HepG2 cell growth takes place in a time- and concentration-dependent manner, that activation of caspase 9 is necessary for PA-induced HepG2 cell apoptosis, that dihydroceramide desaturase 1 (DES1) plays a key role in PA-mediated caspase 9 and caspase 3 activation, and that palmitoleic acid (POA), an omega-7 monounsaturated fatty acid, reverses PA-induced apoptosis through DES1 → Ceramide → Caspase 9 → Caspase 3 signaling.

Keywords: Caspase 9; Cell apoptosis; Ceramide; Dihydroceramide desaturase 1; Palmitic acid; Palmitoleic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis*
  • Caspase 3 / genetics
  • Caspase 3 / metabolism
  • Caspase 9 / genetics
  • Caspase 9 / metabolism*
  • Ceramides / metabolism*
  • Enzyme Activation
  • Hep G2 Cells
  • Humans
  • Oxidoreductases / genetics
  • Oxidoreductases / metabolism*
  • Palmitic Acid / metabolism*

Substances

  • Ceramides
  • Palmitic Acid
  • Oxidoreductases
  • dihydroceramide desaturase
  • Caspase 3
  • Caspase 9