Phorbol ester induces differential membrane-association of protein kinase C subspecies in human platelets

A Fournier; S J Hardy; K J Clark; A W Murray

doi:10.1016/0006-291x(89)92635-1

Phorbol ester induces differential membrane-association of protein kinase C subspecies in human platelets

Biochem Biophys Res Commun. 1989 Jun 15;161(2):556-61. doi: 10.1016/0006-291x(89)92635-1.

Authors

A Fournier¹, S J Hardy, K J Clark, A W Murray

Affiliation

¹ School of Biological Sciences, Flinders University, Bedford Park, South Australia.

PMID: 2735910
DOI: 10.1016/0006-291x(89)92635-1

Abstract

Human platelets contained proteins which cross-reacted with antisera specific for brain protein kinase C-alpha and -beta. When platelets were incubated with 12-O-tetradecanoylphorbol-13-acetate there was a rapid accumulation of protein kinase C-alpha in the particulate fraction associated with a loss of this subspecies from the soluble fraction. No particulate accumulation or soluble loss of protein kinase C-beta could be detected when platelets were incubated with the phorbol ester.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blood Platelets / enzymology*
Blotting, Western
Cell Compartmentation / drug effects
Humans
In Vitro Techniques
Protein Kinase C / blood*
Protein Kinase C / classification
Tetradecanoylphorbol Acetate / pharmacology*

Substances

Protein Kinase C
Tetradecanoylphorbol Acetate