Tumor Cell Detection by Mass Spectrometry Using Signal Ion Emission Reactive Release Amplification

Anal Chem. 2016 Jul 19;88(14):6971-5. doi: 10.1021/acs.analchem.6b02043. Epub 2016 Jun 30.

Abstract

A method is presented for the detection of circulating tumor cells (CTC) using mass spectrometry (MS), through reporter-ion amplification. Particles functionalized with short-chain peptides are bound to cells through antibody-antigen interactions. Selective release and MS detection of peptides is shown to detect as few as 690 cells isolated from a 10 mL blood sample. Here we present proof-of-concept results that pave the way for further investigations.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / chemistry
  • Antibodies / immunology
  • Cell Count
  • Cell Line, Tumor
  • Cell Separation / methods*
  • Humans
  • Immunologic Techniques / methods
  • Limit of Detection
  • Mice
  • Molecular Weight
  • Nanoparticles / chemistry
  • Neoplastic Cells, Circulating* / immunology
  • Peptides / chemistry
  • Silicon Dioxide / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Antibodies
  • Peptides
  • Silicon Dioxide