Abstract
A method is presented for the detection of circulating tumor cells (CTC) using mass spectrometry (MS), through reporter-ion amplification. Particles functionalized with short-chain peptides are bound to cells through antibody-antigen interactions. Selective release and MS detection of peptides is shown to detect as few as 690 cells isolated from a 10 mL blood sample. Here we present proof-of-concept results that pave the way for further investigations.
Publication types
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Letter
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies / chemistry
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Antibodies / immunology
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Cell Count
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Cell Line, Tumor
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Cell Separation / methods*
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Humans
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Immunologic Techniques / methods
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Limit of Detection
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Mice
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Molecular Weight
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Nanoparticles / chemistry
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Neoplastic Cells, Circulating* / immunology
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Peptides / chemistry
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Silicon Dioxide / chemistry
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Substances
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Antibodies
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Peptides
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Silicon Dioxide