Matrix Recrystallization for MALDI-MS Imaging of Maize Lipids at High-Spatial Resolution

Maria Emilia Dueñas; Laura Carlucci; Young Jin Lee

doi:10.1007/s13361-016-1422-0

Matrix Recrystallization for MALDI-MS Imaging of Maize Lipids at High-Spatial Resolution

J Am Soc Mass Spectrom. 2016 Sep;27(9):1575-8. doi: 10.1007/s13361-016-1422-0. Epub 2016 Jun 27.

Authors

Maria Emilia Dueñas^{1

2}, Laura Carlucci¹, Young Jin Lee^{3

4}

Affiliations

¹ Ames Laboratory, US Department of Energy, Ames, IA, 50011, USA.
² Department of Chemistry, Iowa State University of Science and Technology, Ames, IA, 50011, USA.
³ Ames Laboratory, US Department of Energy, Ames, IA, 50011, USA. yjlee@iastate.edu.
⁴ Department of Chemistry, Iowa State University of Science and Technology, Ames, IA, 50011, USA. yjlee@iastate.edu.

PMID: 27349253
DOI: 10.1007/s13361-016-1422-0

Abstract

Matrix recrystallization is optimized and applied to improve lipid ion signals in maize embryos and leaves. A systematic study was performed varying solvent and incubation time. During this study, unexpected side reactions were found when methanol was used as a recrystallization solvent, resulting in the formation of a methyl ester of phosphatidic acid. Using an optimum recrystallization condition with isopropanol, there is no apparent delocalization demonstrated with a transmission electron microscopy (TEM) pattern and maize leaf images obtained at 10 μm spatial resolution. Graphical Abstract ᅟ.

Keywords: High spatial resolution; Lipids; MALDI; Maize; Mass spectrometry imaging; Matrix; Recrystallization.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.