Generation of KCL031 clinical grade human embryonic stem cell line

Stem Cell Res. 2016 Jan;16(1):195-8. doi: 10.1016/j.scr.2015.12.033. Epub 2016 Jan 3.

Abstract

The KCL031 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Blastocyst / cytology
  • Cell Differentiation
  • Cell Line
  • Comparative Genomic Hybridization
  • Genotype
  • Histocompatibility Testing
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / metabolism
  • Humans
  • Karyotype
  • Male
  • Microscopy, Fluorescence
  • Middle Aged

Substances

  • Alkaline Phosphatase