Abstract
Human iPSC line PG64SV.2 was generated from fibroblasts of a patient with a defect of intergenomic communication. This patient harbored a homozygous mutation (c.2243G>C; p.Trp748Ser) in the gene encoding the catalytic subunit of the mitochondrial DNA polymerase gamma gene (POLG). Reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non integrative methodology that involves the use of Sendai virus.
Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cell Differentiation
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Cell Line
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Cellular Reprogramming
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DNA Mutational Analysis
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DNA Polymerase gamma
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DNA-Directed DNA Polymerase / genetics*
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Female
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Humans
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Induced Pluripotent Stem Cells / cytology*
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Induced Pluripotent Stem Cells / metabolism
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Karyotype
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Kruppel-Like Factor 4
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Microscopy, Fluorescence
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Plasmids / metabolism
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Polymorphism, Single Nucleotide
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transfection
Substances
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KLF4 protein, human
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Kruppel-Like Factor 4
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Transcription Factors
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DNA Polymerase gamma
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DNA-Directed DNA Polymerase
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POLG protein, human