Generation of a human control iPSC line with a European mitochondrial haplogroup U background

Stem Cell Res. 2016 Jan;16(1):88-91. doi: 10.1016/j.scr.2015.12.010. Epub 2015 Dec 15.

Abstract

Human iPSC line N44SV.5 was generated from primary normal human dermal fibroblasts belonging to the European mitochondrial haplogroup U. For this purpose, reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus.

Keywords: Defects of intergenomic communication; Induced pluripotent stem cells; POLG; iPS cells; iPSC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cell Line
  • DNA Fingerprinting
  • Europe
  • Haplotypes / genetics*
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Karyotyping
  • Kruppel-Like Factor 4
  • Mitochondria / genetics*