Generation of a human iPSC line from a patient with Leigh syndrome

Stem Cell Res. 2016 Jan;16(1):63-6. doi: 10.1016/j.scr.2015.12.005. Epub 2015 Dec 12.

Abstract

Human iPSC line LND554SV.3 was generated from heteroplasmic fibroblasts of a patient with Leigh syndrome carrying a mutation in the MT-ND5 gene (m.13513GNA; p.D393N). Reprogramming factors Oct3/4, Sox2, Klf4,and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus.

Keywords: Induced pluripotent stem cells; Leigh syndrome; MT-ND5; iPS cells; iPSC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cell Line
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Karyotyping
  • Kruppel-Like Factor 4
  • Leigh Disease / pathology*
  • Sequence Analysis, DNA