Derivation of Genea057 human embryonic stem cell line

Biljana Dumevska; Omar Chami; Robert McKernan; Divya Goel; Uli Schmidt

doi:10.1016/j.scr.2015.11.013

Derivation of Genea057 human embryonic stem cell line

Stem Cell Res. 2016 Jan;16(1):32-6. doi: 10.1016/j.scr.2015.11.013. Epub 2015 Nov 26.

Authors

Biljana Dumevska¹, Omar Chami¹, Robert McKernan¹, Divya Goel¹, Uli Schmidt¹

Affiliation

¹ Genea Biocells, Sydney, Australia.

PMID: 27345782
DOI: 10.1016/j.scr.2015.11.013

Abstract

The Genea057 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated human feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XX karyotype and female allele pattern through traditional karyotyping, CGH and STR analysis. Pluripotency of Genea057 was demonstrated with 97% of cells expressing Nanog, 81% Oct4, 75% Tra1-60 and 97% SSEA4, a PluriTest Pluripotency score of 27.59 and Novelty score of 1.32. The cell line was negative for Mycoplasma and any visible contamination.

MeSH terms

Cell Count
Cell Culture Techniques / methods*
Cell Line / cytology*
Cell Shape
Comparative Genomic Hybridization
Fluorescent Antibody Technique
Human Embryonic Stem Cells / cytology*
Humans
Reproducibility of Results