CRISPR/Cas9: A New Tool for RNA Imaging in Live Cells

Kristina Rau; Andrea Rentmeister

doi:10.1002/cbic.201600342

CRISPR/Cas9: A New Tool for RNA Imaging in Live Cells

Chembiochem. 2016 Sep 15;17(18):1682-4. doi: 10.1002/cbic.201600342. Epub 2016 Jul 22.

Authors

Kristina Rau¹, Andrea Rentmeister²

Affiliations

¹ Institute of Biochemistry, University of Münster, Wilhelm-Klemm-Strasse 2, 48149, Münster, Germany.
² Institute of Biochemistry and, Cells-in-Motion Cluster of Excellence (EXC1003-CiM), University of Münster, Wilhelm-Klemm-Strasse 2, 48149, Münster, Germany. a.rentmeister@uni-muenster.de.

PMID: 27320049
DOI: 10.1002/cbic.201600342

Abstract

Cas9 can be implemented as an RNA-targeting system to track mRNA in living cells. Nuclear export is enabled by efficient targeting of GFP-fused Cas9 to an endogenous mRNA. The approach provides a new and versatile platform for RNA-targeting with applications in RNA imaging and beyond.

Keywords: CRISPR; Cas9; RNA; RNA imaging; RNA tracking; fluorescent probes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Associated Proteins / chemistry
CRISPR-Associated Proteins / metabolism*
CRISPR-Cas Systems*
Cell Nucleus / genetics
Cell Nucleus / metabolism
Green Fluorescent Proteins / chemistry
Green Fluorescent Proteins / metabolism
Molecular Imaging / methods*
RNA / analysis*
RNA / metabolism
RNA Transport*

Substances

CRISPR-Associated Proteins
Green Fluorescent Proteins
RNA