Generation of Resistance to the Diphenyl Ether Herbicide, Oxyfluorfen, via Expression of the Bacillus subtilis Protoporphyrinogen Oxidase Gene in Transgenic Tobacco Plants

K W Choi; O Han; H J Lee; Y C Yun; Y H Moon; M Kim; Y I Kuk; S U Han; J O Guh

doi:10.1271/bbb.62.558

Generation of Resistance to the Diphenyl Ether Herbicide, Oxyfluorfen, via Expression of the Bacillus subtilis Protoporphyrinogen Oxidase Gene in Transgenic Tobacco Plants

Biosci Biotechnol Biochem. 1998;62(3):558-60. doi: 10.1271/bbb.62.558.

Authors

K W Choi¹, O Han², H J Lee³, Y C Yun¹, Y H Moon¹, M Kim², Y I Kuk⁴, S U Han⁴, J O Guh⁴

Affiliations

¹ a Plant Biotechnology Laboratory, Jinro Central Research Institute.
² b Institute of Agricultural Science and Technology, Chonnam National University.
³ c Institute of Biotechnology, Chonnam National University.
⁴ d Department of Agronomy, Chonnam National University.

PMID: 27315932
DOI: 10.1271/bbb.62.558

Abstract

In an effort to develop transgenic plants resistant to diphenyl ether herbicides, we introduced the protoporphyrinogen oxidase (EC 1.3.3.4) gene of Bacillus subtilis into tobacco plants. The results from a Northern analysis and leaf disc assay indicate that the expression of the B. subtilis protoporphyrinogen oxidase gene under the cauliflower mosaic virus 35S promoter generated resistance to the diphenyl ether herbicide, oxyfluorfen, in transgenic tobacco plants.

Keywords: Bacillus subtilis; diphenyl ether herbicide; oxyfluorfen; protoporphyrinogen oxidase; transgenic tobacco plant.