Autophagy induced by snakehead fish vesiculovirus inhibited its replication in SSN-1 cell line

Yao Wang; Nan Chen; Abeer M Hegazy; Xiaodan Liu; Zhixin Wu; Xueqin Liu; Lijuan Zhao; Qiwei Qin; Jiangfeng Lan; Li Lin

doi:10.1016/j.fsi.2016.06.019

Autophagy induced by snakehead fish vesiculovirus inhibited its replication in SSN-1 cell line

Fish Shellfish Immunol. 2016 Aug:55:415-22. doi: 10.1016/j.fsi.2016.06.019. Epub 2016 Jun 14.

Authors

Yao Wang¹, Nan Chen¹, Abeer M Hegazy², Xiaodan Liu¹, Zhixin Wu¹, Xueqin Liu¹, Lijuan Zhao¹, Qiwei Qin³, Jiangfeng Lan⁴, Li Lin⁵

Affiliations

¹ Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
² Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Central Laboratory for Environmental Quality Monitoring (CLEQM), National Water Research Center (NWRC), Cairo, 13621, Egypt.
³ College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
⁴ Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. Electronic address: lanjiangfeng@mail.hzau.edu.cn.
⁵ Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. Electronic address: linli@mail.hzau.edu.cn.

PMID: 27311436
DOI: 10.1016/j.fsi.2016.06.019

Abstract

Autophagy plays an important role in host protection against pathogen infection through activating innate and adaptive immunity. In the present study, we observed that the infection of snakehead fish vesiculovirus (SHVV) could induce apparent autophagy in striped snakehead fish cell line (SSN-1), including clear double-membrane vesicles, fluorescent punctate pattern of microtubule-associated protein 1 light chain 3B (SSN-LC3B) and the conversion of SSN-LC3B-Ⅰ to SSN-LC3B-Ⅱ. Furthermore, we verified that autophagy inhibited the replication of SHVV by assessing mRNA and protein level of nucleoprotein as well as virus titer in the supernatant. These results will shed a new light on the prevention of the infection of SHVV.

Keywords: Antivirus; Autophagy; LC3B; Replication; SHVV.

MeSH terms

Animals
Autophagy*
Cell Line
Perciformes / physiology*
Perciformes / virology*
Vesiculovirus / physiology*
Virus Replication*