The RT-qPCR technique needs a validated set of reference genes for ensuring the consistency of the results from the gene expression. Expression stabilities for 9 genes from Herbaspirillum seropedicae, strain HRC54, grown with different carbon sources were calculated using geNorm and NormFinder, and the gene rpoA showed the best stability values.
Keywords: Carbon source; Expression stability; Herbaspirillum seropedicae; Housekeeping genes; qPCR internal control.
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