Validation of reference genes for RT-qPCR analysis in Herbaspirillum seropedicae

J Microbiol Methods. 2016 Aug:127:193-196. doi: 10.1016/j.mimet.2016.06.011. Epub 2016 Jun 11.

Abstract

The RT-qPCR technique needs a validated set of reference genes for ensuring the consistency of the results from the gene expression. Expression stabilities for 9 genes from Herbaspirillum seropedicae, strain HRC54, grown with different carbon sources were calculated using geNorm and NormFinder, and the gene rpoA showed the best stability values.

Keywords: Carbon source; Expression stability; Herbaspirillum seropedicae; Housekeeping genes; qPCR internal control.

Publication types

  • Validation Study

MeSH terms

  • Carbon / metabolism
  • Genes, Bacterial*
  • Genes, Essential*
  • Herbaspirillum / genetics*
  • Herbaspirillum / metabolism
  • Real-Time Polymerase Chain Reaction / methods*

Substances

  • Carbon