Abstract
The amino acid sequence of protein Gln3 in yeast Saccharomyces cerevisiae has a region enriched with Gln (Q) and Asn (N) residues. In this study, we analyzed the effects of overexpression of Gln3 and its Q/N-rich fragment fused with yellow fluorescent protein (YFP). Being overexpressed, full-length Gln3-YFP does not form aggregates, inhibits vegetative growth, and demonstrates nuclear localization, while the Q/N-rich fragment (Gln3QN) fused with YFP forms aggregates that do not colocalize with the nucleus and do not affect growth of the cells. Although detergent-resistant aggregates of Gln3QN are formed in the absence of yeast prions, the aggregation of Gln3QN significantly increases in the presence of [PIN(+)] prion, while in the presence of two prions, [PSI(+)] and [PIN(+)], the percentage of cells with Gln3QN aggregates is significantly lower than in the strain bearing only [PIN(+)]. Data on colocalization demonstrate that this effect is mediated by interaction between Gln3QN aggregates and [PSI(+)] and [PIN(+)] prions.
MeSH terms
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Amino Acid Sequence
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Asparagine / metabolism*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Cell Nucleus / metabolism
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Glutamine / metabolism*
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Peptide Termination Factors / chemistry
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Peptide Termination Factors / metabolism
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Prions / chemistry
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Prions / metabolism
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / isolation & purification
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / chemistry
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / metabolism*
Substances
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Bacterial Proteins
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GLN3 protein, S cerevisiae
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Luminescent Proteins
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Peptide Termination Factors
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Prions
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RNQ1 protein, S cerevisiae
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Recombinant Fusion Proteins
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SUP35 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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yellow fluorescent protein, Bacteria
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Glutamine
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Asparagine