Identification of Lactobacillus brevis using a species-specific AFLP-derived marker

Int J Food Microbiol. 2016 Sep 2:232:90-4. doi: 10.1016/j.ijfoodmicro.2016.06.002. Epub 2016 Jun 4.

Abstract

A simple and specific method for the rapid detection and identification of Lactobacillus brevis was developed. A fAFLP (Fluorescent Amplified Fragment Length Polymorphisms) marker for L. brevis was used to design oligonucleotide primers for a species-specific PCR assay, targeting a 125bp fragment of the gene encoding the aldo/keto reductase of the diketogulonate-reductase family of L. brevis. This assay resulted in 100% inclusivity and exclusivity of assignment of strains to the species L. brevis. The analytical specificity of this assay was successfully tested to identify L. brevis isolates from sourdoughs.

Keywords: Lactic acid bacteria; Lactobacillus brevis; Microbial food quality; Sourdough; Species identification.

MeSH terms

  • Aldehyde Reductase / genetics
  • Aldo-Keto Reductases
  • Amplified Fragment Length Polymorphism Analysis / methods*
  • Base Sequence
  • Bread / microbiology
  • Cheese / microbiology
  • DNA Primers / genetics
  • DNA, Bacterial / genetics*
  • Fermentation
  • Levilactobacillus brevis / classification
  • Levilactobacillus brevis / enzymology
  • Levilactobacillus brevis / genetics*
  • Meat / microbiology
  • Molecular Typing / methods*
  • Polymerase Chain Reaction / methods*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Species Specificity

Substances

  • DNA Primers
  • DNA, Bacterial
  • RNA, Ribosomal, 16S
  • Aldo-Keto Reductases
  • Aldehyde Reductase