Transport mechanism of a glutamate transporter homologue GltPh

Yurui Ji; Vincent L G Postis; Yingying Wang; Mark Bartlam; Adrian Goldman

doi:10.1042/BST20160055

Transport mechanism of a glutamate transporter homologue GltPh

Biochem Soc Trans. 2016 Jun 15;44(3):898-904. doi: 10.1042/BST20160055.

Authors

Yurui Ji¹, Vincent L G Postis², Yingying Wang³, Mark Bartlam⁴, Adrian Goldman⁵

Affiliations

¹ College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, LS2 9JT, U.K.
² Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, LS2 9JT, U.K. Biomedicine Research Group, Faculty of Health and Social Sciences, Leeds Beckett University, Leeds, LS1 3JT, U.K.
³ College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.
⁴ Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, LS2 9JT, U.K. College of Life Sciences, Nankai University, Tianjin 300071, China bartlam@nankai.edu.cn a.goldman@leeds.ac.uk.
⁵ Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, LS2 9JT, U.K. College of Life Sciences, Nankai University, Tianjin 300071, China Division of Biochemistry, Department of Biosciences, University of Helsinki, Helsinki, FIN-00014, Finland bartlam@nankai.edu.cn a.goldman@leeds.ac.uk.

Abstract

Glutamate transporters are responsible for uptake of the neurotransmitter glutamate in mammalian central nervous systems. Their archaeal homologue GltPh, an aspartate transporter isolated from Pyrococcus horikoshii, has been the focus of extensive studies through crystallography, MD simulations and single-molecule FRET (smFRET). Here, we summarize the recent research progress on GltPh, in the hope of gaining some insights into the transport mechanism of this aspartate transporter.

Keywords: Cl− conductance; GltPh; Na+ coupling; aspartate transporter; excitatory amino acid transporters (EAATs); glutamate transporter.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Transport System X-AG / metabolism*
Aspartic Acid / metabolism
Biological Transport
Protein Conformation
Pyrococcus horikoshii / metabolism*
Substrate Specificity

Substances

Amino Acid Transport System X-AG
Aspartic Acid

Abstract

Publication types

MeSH terms

Substances

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