(1)H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization

Enrico Ravera; Linda Cerofolini; Tommaso Martelli; Alexandra Louka; Marco Fragai; Claudio Luchinat

doi:10.1038/srep27851

(1)H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization

Sci Rep. 2016 Jun 9:6:27851. doi: 10.1038/srep27851.

Authors

Enrico Ravera¹, Linda Cerofolini^{1

2}, Tommaso Martelli³, Alexandra Louka¹, Marco Fragai^{1

2}, Claudio Luchinat^{1

2

3}

Affiliations

¹ Magnetic Resonance Center (CERM), University of Florence, and Interuniversity Consortium for Magnetic Resonance of Metalloproteins (CIRMMP), Via L. Sacconi 6, 50019 Sesto Fiorentino (FI), Italy.
² Department of Chemistry "Ugo Schiff", University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy.
³ Giotto Biotech S.R.L., Via Madonna del Piano 6, 50019 Sesto Fiorentino (FI), Italy.

Abstract

Proton-detection in solid-state NMR, enabled by high magnetic fields (>18 T) and fast magic angle spinning (>50 kHz), allows for the acquisition of traditional (1)H-(15)N experiments on systems that are too big to be observed in solution. Among those, proteins entrapped in a bioinspired silica matrix are an attractive target that is receiving a large share of attention. We demonstrate that (1)H-detected SSNMR provides a novel approach to the rapid assessment of structural integrity in proteins entrapped in bioinspired silica.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Nuclear Magnetic Resonance, Biomolecular / instrumentation
Nuclear Magnetic Resonance, Biomolecular / methods*
Protons*
Silicon Dioxide / chemistry*

Substances

Protons
Silicon Dioxide