A quantitative headspace-solid-phase microextraction-gas chromatography-flame ionization detector method to analyze short chain free fatty acids in rat feces

Anal Biochem. 2016 Sep 1:508:12-4. doi: 10.1016/j.ab.2016.05.023. Epub 2016 Jun 4.

Abstract

This study sought to develop and validate a quantitative method to analyze short chain free fatty acids (SCFAs) in rat feces by solid-phase microextraction and gas chromatography (SPME-GC) using the salt mixture ammonium sulfate and sodium dihydrogen phosphate as salting out agent. Conditioning and extraction time, linearity, limits of detection and quantification, repeatability, and recovery were evaluated. The proposed method allows quantification with improved sensitivity as compared with other methods exploiting SPME-GC. The method has been applied to analyze rat fecal samples, quantifying acetic, propionic, isobutyric, butyric, isopentanoic, pentanoic, and hexanoic acids.

Keywords: Rat feces; Short chain free fatty acids; Solid-phase microextraction.

Publication types

  • Validation Study

MeSH terms

  • Animals
  • Chemistry Techniques, Analytical / instrumentation*
  • Chemistry Techniques, Analytical / methods*
  • Chromatography, Gas*
  • Fatty Acids, Nonesterified / analysis*
  • Feces / chemistry*
  • Flame Ionization*
  • Limit of Detection
  • Rats
  • Reproducibility of Results
  • Solid Phase Microextraction*

Substances

  • Fatty Acids, Nonesterified