Maintenance of High Cytochrome P450 Expression in HepaRG Cell Spheroids in DMSO-Free Medium

A V Aleksandrova; O A Burmistrova; K A Fomicheva; D A Sakharov

doi:10.1007/s10517-016-3360-z

Maintenance of High Cytochrome P450 Expression in HepaRG Cell Spheroids in DMSO-Free Medium

Bull Exp Biol Med. 2016 May;161(1):120-4. doi: 10.1007/s10517-016-3360-z. Epub 2016 Jun 6.

Authors

A V Aleksandrova¹, O A Burmistrova², K A Fomicheva³, D A Sakharov^{2

4}

Affiliations

¹ BioClinicum Research and Production Center, Moscow, Russia. a.aleksandrova@bioclinicum.com.
² BioClinicum Research and Production Center, Moscow, Russia.
³ National Medical Research Radiology Center, Moscow, Russia.
⁴ Moscow State University of Mechanical Engineering (MAMI), Moscow, Russia.

PMID: 27265124
DOI: 10.1007/s10517-016-3360-z

Abstract

We studied the effects of DMSO and fibroblasts during HepaRG cell spheroid formation and conditions of their subsequent culturing on the levels of mRNA of the major cytochromes P450. A protocol of spheroid formation from differentiated HepaRG cells and their culturing in serum- and DMSO-free medium is developed.

Keywords: HepaRG; cytochromes P450; differentiation.

MeSH terms

Cell Line
Culture Media / chemistry
Cytochrome P-450 Enzyme System / genetics*
Cytochrome P-450 Enzyme System / metabolism
Dimethyl Sulfoxide / chemistry
Gene Expression
Gene Expression Regulation, Enzymologic
Hepatocytes / enzymology*
Humans
RNA, Messenger / genetics
RNA, Messenger / metabolism
Spheroids, Cellular / enzymology*

Substances

Culture Media
RNA, Messenger
Cytochrome P-450 Enzyme System
Dimethyl Sulfoxide