The expression of MYH9 in osteosarcoma and its effect on the migration and invasion abilities of tumor cell

Wei Zhou; Ming-Yu Fan; You-Xiu Wei; Song Huang; Jing-You Chen; Ping Liu

doi:10.1016/j.apjtm.2016.04.011

The expression of MYH9 in osteosarcoma and its effect on the migration and invasion abilities of tumor cell

Asian Pac J Trop Med. 2016 Jun;9(6):597-600. doi: 10.1016/j.apjtm.2016.04.011. Epub 2016 Apr 19.

Authors

Wei Zhou¹, Ming-Yu Fan², You-Xiu Wei¹, Song Huang¹, Jing-You Chen¹, Ping Liu³

Affiliations

¹ Department of Orthopaedics, Liyuan Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430077, Hubei, China.
² Wuhan Third Hospital, Wuhan, 430000, Hubei, China.
³ Department of Orthopaedics, Liyuan Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430077, Hubei, China. Electronic address: 136477198@qq.com.

PMID: 27262074
DOI: 10.1016/j.apjtm.2016.04.011

Abstract

Objective: To determine the expression of non-muscle myosin heavy chain 9 (MYH9) in osteosarcoma and its effect on the migration and invasion abilities of tumor cell.

Methods: A total of 65 cases of osteosarcoma and 20 cases with benign osteochondroma who underwent resection operation in the Orthopaedics Department of our hospital from January 1st 2009 to January 1st 2015 were selected. Their mRNA levels of MYH9 were tested by qrt-PCR. Immunohistochemical method was used to examine the expression of MYH9 in osteosarcoma and the correlation between the positive expression of MYH9 and the clinicopathological features of patients was illustrated by statistical analysis. MYH9 was compounded artificially. The expression of MYH9 in SAOS2 osteosarcoma cells was decreased by siRNA. Scratch test was used to determine the change of SAOS2 cell migration ability after MYH9 silence. Transwell assay was employed to detect the change of cell invasion ability after MYH9 silence.

Results: The expression levels of mRNA of MYH9 and protein in osteosarcoma tissues were significantly higher than those in benign osteochondroma tissues. The high expression of MYH9 in osteosarcoma tissues was apparently related to the high Enneking classification (III classification) and lung metastasis. SiRNA of MYH9 could evidently decrease the expression level of MYH9 in SAOS2. The down-regulated expression of MYH9 could inhibit the migration and invasion abilities of SAOS2 cells.

Conclusions: MYH9 shows a trend of high expression in osteosarcoma tissues, and its high expression is associated with features such as tumor invasion and metastasis. The down-regulated MYH9 can realize an anti-tumor effect by inhibiting the migration and invasion of osteosarcoma cells.

Keywords: Clinical significance; Invasion; MYH9; Migration; Osteosarcoma.