Involvement of α5 integrin in survivin-mediated osteosarcoma metastasis

Xiao-Zhou Liu; Cheng-Jun Li; Su-Jia Wu; Xin Shi; Jian-Ning Zhao

doi:10.1016/j.apjtm.2016.03.022

Involvement of α5 integrin in survivin-mediated osteosarcoma metastasis

Asian Pac J Trop Med. 2016 May;9(5):478-83. doi: 10.1016/j.apjtm.2016.03.022. Epub 2016 Mar 23.

Authors

Xiao-Zhou Liu¹, Cheng-Jun Li², Su-Jia Wu², Xin Shi², Jian-Ning Zhao³

Affiliations

¹ Southern Medical University, Guangzhou 510515, China; Department of Orthopedics, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.
² Department of Orthopedics, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.
³ Southern Medical University, Guangzhou 510515, China; Southern Medical University, Guangzhou 510515, Guangdong Province, China. Electronic address: zhaojianning.0207@163.com.

PMID: 27261858
DOI: 10.1016/j.apjtm.2016.03.022

Abstract

Objective: To investigate the role of survivin in osteosarcoma metastasis.

Methods: Small interfering RNA (siRNA) was used to knockdown the expression of survivin and α5 integrin in the human osteosarcoma cell line MG63. Western blotting and immunostaining methods were used to assessed the effect of survivin knockdown on the expression of α5 integrin through flow cytometry and fluorescence microscopy detection. Meanwhile, the invasion and migration of transfected cells in Transwell and wound healing assays were probed, and the growth situation of these cells transplanted into nude mice was monitored.

Results: Knockdown of survivin expression could inhibit the invasion and migration of osteosarcoma MG64 cells in vitro and the expression of α5 integrin on osteosarcoma MG64 cell surface, suggesting that survivin can inhibit the invasion and migration of osteosarcoma cells through downregulation of α5 integrin. Anti-α5 integrin antibody could also markedly decrease the capability of invasion and migration of osteosarcoma MG64 cells. Additionally, knockdown of survivin expression could slow the growth of osteosarcoma MG63 cells transplanted into nude mice.

Conclusions: Survivin-directed anti-tumor strategies might be an effective method in the treatment of osteosarcoma.

Keywords: Metastasis; Osteosarcoma; Survivin; α5 integrin.