pH and temperature stability of (-)-epigallocatechin-3-gallate-β-cyclodextrin inclusion complex-loaded chitosan nanoparticles

Fei Liu; Hamid Majeed; John Antoniou; Yue Li; Yun Ma; Wallace Yokoyama; Jianguo Ma; Fang Zhong

doi:10.1016/j.carbpol.2016.04.100

pH and temperature stability of (-)-epigallocatechin-3-gallate-β-cyclodextrin inclusion complex-loaded chitosan nanoparticles

Carbohydr Polym. 2016 Sep 20:149:340-7. doi: 10.1016/j.carbpol.2016.04.100. Epub 2016 Apr 28.

Authors

Fei Liu¹, Hamid Majeed², John Antoniou², Yue Li², Yun Ma², Wallace Yokoyama³, Jianguo Ma², Fang Zhong⁴

Affiliations

¹ State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, People's Republic of China; Western Regional Research Center, ARS, U.S. Department of Agriculture, Albany, CA 94710, United States.
² State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, People's Republic of China.
³ Western Regional Research Center, ARS, U.S. Department of Agriculture, Albany, CA 94710, United States.
⁴ State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, People's Republic of China. Electronic address: fzhong@jiangnan.edu.cn.

PMID: 27261758
DOI: 10.1016/j.carbpol.2016.04.100

Abstract

The oxidative stability of (-)-epigallocatechin-3-gallate (EGCG) incorporated as inclusion complexes (ICs) in sulfobutylether-β-cyclodextrin sodium (SBE-β-CD) and then ionotropically crosslinked with chitosan hydrochloride (CSH) into nanoparticles were investigated. EGCG-loaded CSH-SBE-β-CD nanoparticles (CSNs) were physically unstable at higher pH and temperature. The particle size of CSNs was unchanged in the pH range of 3-5, but the microenvironment of EGCG-IC appeared to be intact until the pH increased to 6.5 by fluorescence spectroscopy. The physical structure of EGCG-ICs was also affected during storage in addition to CSNs, which was further affected as temperature increased from 25 to 55°C. The decrease in antioxidant activities of EGCG-ICs and free EGCG with increasing pH, storage time and temperature were modest compared to the prominent decreases in antioxidant activities of EGCG-loaded CSNs. The extreme entrapment of EGCG-ICs and/or free EGCG in the aggregated CSNs restricted the release of EGCG, thus inhibiting the antioxidant activities.

Keywords: (−)-Epigallocatechin-3-gallate; (−)-Epigallocatechin-3-gallate (PubChem CID: 65064); Antioxidant activity; Chitosan hydrochloride (PubChem CID: 71853); Chitosan nanoparticles; Inclusion complexes; Stability; Sulfobutylether-β-cyclodextrin; Sulfobutylether-β-cyclodextrin sodium (PubChem CID: 66577045).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Catechin / analogs & derivatives*
Catechin / chemistry
Chitosan / chemistry*
Drug Carriers / chemistry*
Drug Stability
Hydrogen-Ion Concentration
Nanoparticles / chemistry*
Particle Size
Temperature*
beta-Cyclodextrins / chemistry*

Substances

Drug Carriers
beta-Cyclodextrins
SBE4-beta-cyclodextrin
Catechin
Chitosan
epigallocatechin gallate