Genome sequencing and annotation of a Campylobacter coli strain isolated from milk with multidrug resistance

Kun C Liu; Karen C Jinneman; Jason Neal-McKinney; Wen-Hsin Wu; Daniel H Rice

doi:10.1016/j.gdata.2016.05.003

Genome sequencing and annotation of a Campylobacter coli strain isolated from milk with multidrug resistance

Genom Data. 2016 May 5:8:123-5. doi: 10.1016/j.gdata.2016.05.003. eCollection 2016 Jun.

Authors

Kun C Liu¹, Karen C Jinneman¹, Jason Neal-McKinney¹, Wen-Hsin Wu¹, Daniel H Rice¹

Affiliation

¹ Applied Technology Center, Pacific Regional Laboratory Northwest, US Food and Drug Administration, 22201 23rd Drive SE, Bothell, WA 98012, United States.

Abstract

As the most prevalent bacterial cause of human gastroenteritis, food-borne Campylobacter infections pose a serious threat to public health. Whole Genome Sequencing (WGS) is a tool providing quick and inexpensive approaches for analysis of food-borne pathogen epidemics. Here we report the WGS and annotation of a Campylobacter coli strain, FNW20G12, which was isolated from milk in the United States in 1997 and carries multidrug resistance. The draft genome of FNW20G12 (DDBJ/ENA/GenBank accession number LWIH00000000) contains 1, 855,435 bp (GC content 31.4%) with 1902 annotated coding regions, 48 RNAs and resistance to aminoglycoside, beta-lactams, tetracycline, as well as fluoroquinolones. There are very few genome reports of C. coli from dairy products with multidrug resistance. Here the draft genome of FNW20G12, a C. coli strain isolated from raw milk, is presented to aid in the epidemiology study of C. coli antimicrobial resistance and role in foodborne outbreak.

Keywords: C. coli (Campylobacter coli); RAST (Rapid Annotation using Subsystem Technology); WGS (Whole Genome Sequencing).