Abstract
Four new steroids, namely klyflaccisteroids G-J (1-4) were isolated from the Formosan soft coral Klyxum flaccidum. The structures of compounds 1-4 were established by spectral data analysis (IR, MS, 1D and 2D NMR) and comparison of spectral data with those of the related known compounds. Cytotoxicity assay revealed that 4 exhibited inhibition activity against the growth of HT-29, P388 and K562 cancer cell lines, whereas 2 showed selective cytotoxicity toward P388 cells. Compound 4 was also found to display significant anti-inflammatory activity for suppressing superoxide anion generation (O2(-)) and elastase release.
Keywords:
Anti-inflammatory activity; Cytotoxicity; Klyxum flaccidum; Soft coral; Steroid.
Copyright © 2016 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anthozoa / chemistry*
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / isolation & purification
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Antineoplastic Agents / pharmacology*
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Dose-Response Relationship, Drug
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Drug Screening Assays, Antitumor
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Humans
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Mice
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Molecular Structure
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Pancreatic Elastase / antagonists & inhibitors*
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Pancreatic Elastase / metabolism
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Steroids / chemistry
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Steroids / isolation & purification
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Steroids / pharmacology*
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Structure-Activity Relationship
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Superoxides / antagonists & inhibitors*
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Superoxides / metabolism
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Antineoplastic Agents
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Steroids
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Superoxides
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Pancreatic Elastase