Using Multiple Whole-Cell Recordings to Study Spike-Timing-Dependent Plasticity in Acute Neocortical Slices

Txomin Lalanne; Therese Abrahamsson; P Jesper Sjöström

doi:10.1101/pdb.prot091306

Using Multiple Whole-Cell Recordings to Study Spike-Timing-Dependent Plasticity in Acute Neocortical Slices

Cold Spring Harb Protoc. 2016 Jun 1;2016(6):pdb.prot091306. doi: 10.1101/pdb.prot091306.

Authors

Txomin Lalanne¹, Therese Abrahamsson², P Jesper Sjöström²

Affiliations

¹ Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montréal General Hospital, Montréal, Québec H3G 1A4, Canada Integrated Program in Neuroscience, McGill University, 3801 University Street, Montréal, Quebec H3A 2B4, Canada.
² Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montréal General Hospital, Montréal, Québec H3G 1A4, Canada.

Abstract

This protocol provides a method for quadruple whole-cell recording to study synaptic plasticity of neocortical connections, with a special focus on spike-timing-dependent plasticity (STDP). It also describes how to morphologically identify recorded cells from two-photon laser-scanning microscopy (2PLSM) stacks.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
In Vitro Techniques / methods*
Neocortex / physiology*
Neuronal Plasticity*
Patch-Clamp Techniques / methods*
Rodentia

Abstract

Publication types

MeSH terms

Grants and funding