Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues

Laura Bindila; Beat Lutz

doi:10.1007/978-1-4939-3539-0_2

Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues

Methods Mol Biol. 2016:1412:9-18. doi: 10.1007/978-1-4939-3539-0_2.

Authors

Laura Bindila¹, Beat Lutz²

Affiliations

¹ Institute for Physiological Chemistry, University Medical Center, Duesbergweg 6, 55128, Mainz, Germany.
² Institute for Physiological Chemistry, University Medical Center, Duesbergweg 6, 55128, Mainz, Germany. beat.lutz@uni-mainz.de.

PMID: 27245887
DOI: 10.1007/978-1-4939-3539-0_2

Abstract

Extraction and quantification of endocannabinoids (eCBs) from biological tissues are essential to unravel their changes in physiological and pathophysiological conditions. We describe here an analytical protocol for extraction of endocannabinoids, anandamide (AEA) and 2-arachidonoyl glycerol (2-AG), endocannabinoid-like lipids such as palmitoyl ethanolamide (PEA) and oleoyl ethanolamide (OEA), as well as arachidonic acid (AA) from biological tissues using liquid-liquid extraction method and simultaneous quantification by liquid chromatography multiple reaction monitoring (LC/MRM).

Keywords: Endocannabinoid-like lipids; Endocannabinoids; Lipid extraction; Liquid-chromatography; Multiple reaction monitoring.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid* / methods
Endocannabinoids / chemistry*
Endocannabinoids / isolation & purification*
Lipids / chemistry*
Lipids / isolation & purification*
Liquid-Liquid Extraction* / methods

Substances

Endocannabinoids
Lipids