Methods for determining the antimicrobial susceptibility of mycobacteria
Enferm Infecc Microbiol Clin. 2017 Oct;35(8):529-535.
doi: 10.1016/j.eimc.2016.04.008.
Epub 2016 May 25.
[Article in
English,
Spanish]
Affiliations
- 1 Servicio de Microbiología, Hospital Universitari de Bellvitge, Departament de Patologia i Terapèutica Experimental, Universitat de Barcelona. GEIM (SEIMC), Hospitalet de Llobregat, Barcelona, España. Electronic address: falcaide@bellvitgehospital.cat.
- 2 Departamento de Microbiología Clínica, IIS-Fundación Jiménez Díaz, Universidad Autónoma de Madrid (UAM). GEIM (SEIMC), Madrid, España.
- 3 Servei de Microbiologia-CDB, Hospital Clínic-ISGLOBAL, Departament de Fonaments Clínics, Facultat de Medicina, Universitat de Barcelona. GEIM (SEIMC), Barcelona, España.
- 4 Unidad de Referencia Regional de Micobacterias, Servicio de Microbiología, Hospital Universitario Central de Asturias. GEIM (SEIMC), Oviedo, Asturias, España.
Abstract
Mycobacteria are a large group of microorganisms, multiple species of which are major causes of morbidity and mortality, such as tuberculosis and leprosy. At present, the emergence and spread of multidrug-resistant strains of Mycobacterium tuberculosis complex are one of the most serious health problems worldwide. Furthermore, in contrast to M. tuberculosis and Mycobacterium leprae, non-tuberculous mycobacteria (NTM) are more frequently isolated and, in many cases, treatment is based on drug susceptibility testing. This article is a review of the different methods to determine the in vitro drug susceptibility of M. tuberculosis complex and the most relevant NTM isolates. The molecular techniques currently used for rapid detection of resistance of clinical specimens are also analysed.
Keywords:
Mecanismos de resistencia; Micobacterias; Mycobacteria; Prueba de sensibilidad; Resistance mechanisms; Susceptibility testing.
Copyright © 2016 Elsevier España, S.L.U. y Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Antitubercular Agents / pharmacology
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Drug Resistance, Microbial*
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Drug Resistance, Multiple, Bacterial
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Humans
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Microbial Sensitivity Tests / methods*
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Molecular Diagnostic Techniques
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Mycobacterium / classification
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Mycobacterium / drug effects*
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Mycobacterium Infections / microbiology
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Nontuberculous Mycobacteria / drug effects
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Species Specificity
Substances
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Anti-Bacterial Agents
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Antitubercular Agents