Molecular Epidemiology of Chikungunya Virus by Sequencing

Methods Mol Biol. 2016:1426:11-9. doi: 10.1007/978-1-4939-3618-2_2.

Abstract

Molecular surveillance of Chikungunya virus (CHIKV) is important as it provides data on the circulating CHIKV genotypes in endemic countries and enabling activation of measures to be taken in the event of a pending outbreak. Molecular surveillance is carried out by first detecting CHIKV in susceptible humans or among field-caught mosquitoes. This is followed by sequencing a selected region of the virus which will provide evidence on the source of the virus and possible association of the virus to increased cases of Chikungunya infections.

Keywords: Molecular surveillance; Phylogenetic tree; RT-PCR; Sequencing.

MeSH terms

  • Animals
  • Cell Line
  • Chikungunya Fever / diagnosis*
  • Chikungunya Fever / epidemiology*
  • Chikungunya virus / classification
  • Chikungunya virus / genetics*
  • Chikungunya virus / isolation & purification
  • Culicidae / virology*
  • Disease Outbreaks
  • Humans
  • Molecular Epidemiology
  • Phylogeny
  • Population Surveillance
  • Sequence Analysis, RNA
  • Viral Envelope Proteins / genetics

Substances

  • Viral Envelope Proteins