Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques

Annalisa D'Arco; Nadia Brancati; Maria Antonietta Ferrara; Maurizio Indolfi; Maria Frucci; Luigi Sirleto

doi:10.1364/BOE.7.001853

Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques

Biomed Opt Express. 2016 Apr 15;7(5):1853-64. doi: 10.1364/BOE.7.001853. eCollection 2016 May 1.

Authors

Annalisa D'Arco¹, Nadia Brancati², Maria Antonietta Ferrara³, Maurizio Indolfi³, Maria Frucci², Luigi Sirleto³

Affiliations

¹ National Research Council (CNR), Institute for Microelectronics and Microsystems, I-80131 Napoli, Italy; Second University of Naples (SUN), Department of Information Engineering, I-81031 Aversa, Italy.
² National Research Council (CNR), Institute for High-Performance Computing and Networking, I-80131 Napoli, Italy.
³ National Research Council (CNR), Institute for Microelectronics and Microsystems, I-80131 Napoli, Italy.

Abstract

The visualization of heterogeneous morphology, segmentation and quantification of image features is a crucial point for nonlinear optics microscopy applications, spanning from imaging of living cells or tissues to biomedical diagnostic. In this paper, a methodology combining stimulated Raman scattering microscopy and image analysis technique is presented. The basic idea is to join the potential of vibrational contrast of stimulated Raman scattering and the strength of imaging analysis technique in order to delineate subcellular morphology with chemical specificity. Validation tests on label free imaging of polystyrene-beads and of adipocyte cells are reported and discussed.

Keywords: (100.0100) Image processing; (170.0110) Imaging systems; (180.4315) Nonlinear microscopy; (190.5890) Scattering, stimulated; (190.7110) Ultrafast nonlinear optics.