Complete Genome Sequence of Pseudorabies Virus Reference Strain NIA3 Using Single-Molecule Real-Time Sequencing

Elisabeth Mathijs; Frank Vandenbussche; Sara Verpoest; Nick De Regge; Steven Van Borm

doi:10.1128/genomeA.00440-16

Complete Genome Sequence of Pseudorabies Virus Reference Strain NIA3 Using Single-Molecule Real-Time Sequencing

Genome Announc. 2016 May 26;4(3):e00440-16. doi: 10.1128/genomeA.00440-16.

Authors

Elisabeth Mathijs¹, Frank Vandenbussche², Sara Verpoest³, Nick De Regge³, Steven Van Borm²

Affiliations

¹ Molecular Platform, Veterinary and Agrochemical Research Centre, Ukkel, Belgium elisabeth.mathijs@coda-cerva.be.
² Molecular Platform, Veterinary and Agrochemical Research Centre, Ukkel, Belgium.
³ Enzootic and (Re)emerging Diseases, Veterinary and Agrochemical Research Centre, Ukkel, Belgium.

Abstract

Pseudorabies virus (PRV) is the causative agent of Aujeszky's disease in pigs. PRV strains are also used as model organisms for the study of alphaherpesvirus biology or for neuronal pathway studies. We present here the complete genome of the virulent wild-type PRV reference strain NIA3, determined by single-molecule real-time sequencing.

Grants and funding

This work was supported by the European Union FP7 project RAPIDIA-FIELD (grant number FP7-289364) and Epi-SEQ, a transnational research project supported under the 2nd joint call for transnational research projects by EMIDA ERA-NET (FP7 project no. 219235).