pH-Activated Targeting Drug Delivery System Based on the Selective Binding of Phenylboronic Acid

ACS Appl Mater Interfaces. 2016 Jun 15;8(23):14845-54. doi: 10.1021/acsami.6b04737. Epub 2016 Jun 3.

Abstract

Phenylboronic acid (PBA) is a tumor-targeting molecule, but its nonspecific interaction with normal cells or other components containing cis-diol residues undoubtedly limits its potential application in tumor-targeting drug delivery. Herein, we developed fructose-coated mixed micelles via PBA-terminated polyethylene glycol monostearate (PBA-PEG-C18) and Pluronic P123 (PEG20-PPG70-PEG20) to solve this problem, as the stability of borate formed by PBA and fructose was dramatically dependent on pH. The fluorescence spectroscopic results indicated that the borate formed by PBA and fructose decomposed at a decreased pH, and better binding between PBA and sialic acid (SA) was observed at a low pH. These results implied that the fructose groups decorated on the surface of the micelles could be out-competed by SA at a low pH. In vitro uptake and cytotoxicity studies demonstrated that the fructose coating on the mixed micelles improved the endocytosis and enhanced the cytotoxicity of drug-loaded mixed micelles in HepG2 cells but reduced the cytotoxicity in normal cells. These results demonstrate that a simple decorating strategy may facilitate PBA-targeted nanoparticles for tumor-specific drug delivery.

Keywords: competitive binding; drug delivery; pH activation; phenylboronic acid; target.

MeSH terms

  • Antineoplastic Agents / administration & dosage
  • Boronic Acids / chemistry
  • Boronic Acids / metabolism*
  • Boronic Acids / toxicity
  • Cell Survival / drug effects
  • Drug Delivery Systems / methods*
  • Hep G2 Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Micelles
  • Neoplasms / drug therapy
  • Polyethylene Glycols

Substances

  • Antineoplastic Agents
  • Boronic Acids
  • Micelles
  • Polyethylene Glycols
  • benzeneboronic acid