Status and transport of water in plant body are the main contents of study of soil-plant-atmosphere continuum (SPAC), as well as the base for use and regulation of agricultural water. The process of water transport in plant can be deeply influenced by the environments. Thus, plant needs to adjust its water status to accommodate the environmental change to sustain its own growth and development. Traditional methods for plant water monitoring, such as evaporation flux, pressure chamber, high pressure flow meter, heat pulse, and so on, usually cause damage or even destruction of plant body and disturb the original water status. Thus, they are not able to truly and precisely detect and reflect the real water status of plant. Nuclear magnetic resonance (NMR) is a non-destructive and non-invasive technique which can be used for the measurement of water molecular displacement, and transportation. This study aimed to provide an overview of the applications of NMR technique in the study of water distribution and transport in plant roots and stems, as well as the water content in plant cells and tissues. In addition, the existing main problems and possible solutions were analyzed for the applications of NMR in SPAC studies. Several important issues were proposed for the acquisition of more precise and reliable detection signals. It was suggested that the NMR technique would probably make important progress in the relevant fields such as plant water physiology, plantenvironment interactions, and water metabolism. In general, the application of NMR in SPAC system study was still in its infancy in China. The deeper application and expansion of NMR in SPAC study would depend on the development of portable and open NMR equipment that could be easily applied for different plants in field.