Validation data for the quantification of the Annonaceous acetogenin annonacin in Rat brain by UPLC-MS/MS

Natacha Bonneau; Isabelle Schmitz-Afonso; Alain Brunelle; David Touboul; Pierre Champy

doi:10.1016/j.dib.2016.04.067

Validation data for the quantification of the Annonaceous acetogenin annonacin in Rat brain by UPLC-MS/MS

Data Brief. 2016 May 4:7:1633-8. doi: 10.1016/j.dib.2016.04.067. eCollection 2016 Jun.

Authors

Natacha Bonneau¹, Isabelle Schmitz-Afonso², Alain Brunelle², David Touboul², Pierre Champy¹

Affiliations

¹ Laboratoire de Pharmacognosie, BioCIS, Univ. Paris-Sud, CNRS, Université Paris-Saclay, UFR Pharmacie, 5 rue J.-B. Clément, 92290 Châtenay-Malabry, France.
² Institut de Chimie des Substances Naturelles, CNRS UPR2301, Université Paris-Saclay, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.

Abstract

Annonaceous acetogenins (AAGs) are environmental neurotoxins from the fruit pulp of several Annonaceae species, whose consumption was linked to the occurrence of sporadic atypical Parkinsonism with dementia. The quantification of the prototypical AAG annonacin in Rat brain homogenates was performed by UPLC-MS/MS in selected reaction monitoring (SRM) mode, using a triple quadrupole mass analyzer. A natural analog of annonacin was used as an internal standard. Analyzed data set of the analytical validation of this method is presented, including stability of the samples, extraction recovery and matrix effect, supporting the results described in the article "Quantification of the environmental neurotoxin annonacin in Rat brain by UPLC-MS/MS" N. Bonneau, I. Schmitz-Afonso, D. Touboul, A. Brunelle, P. Champy (2016) [1].

Keywords: AAG, Annonaceous acetogenin; EMA, European Medicines Agency; IS, internal standard; LLOQ, lower limit of quantification; MF, matrix factor; MQC, medium concentration quality control; QC, quality control; RSD, relative standard deviation; SRM, selected reaction monitoring; ULOQ, upper limit of quantification; UPLC-MS/MS, ultra-performance liquid chromatography tandem mass spectrometry; amu, atomic mass unit.