Microwave-assisted hydrothermal extraction of sulfated polysaccharides from Ulva spp. and Monostroma latissimum

Shuntaro Tsubaki; Kiriyo Oono; Masanori Hiraoka; Ayumu Onda; Tomohiko Mitani

doi:10.1016/j.foodchem.2016.04.121

Microwave-assisted hydrothermal extraction of sulfated polysaccharides from Ulva spp. and Monostroma latissimum

Food Chem. 2016 Nov 1:210:311-6. doi: 10.1016/j.foodchem.2016.04.121. Epub 2016 Apr 26.

Authors

Shuntaro Tsubaki¹, Kiriyo Oono², Masanori Hiraoka³, Ayumu Onda⁴, Tomohiko Mitani⁵

Affiliations

¹ Oceanography Section, Science Research Center, Kochi University, Akebono-cho 2-5-1, Kochi City, Kochi 780-8520, Japan. Electronic address: shuntaro.tsubaki@gmail.com.
² Oceanography Section, Science Research Center, Kochi University, Akebono-cho 2-5-1, Kochi City, Kochi 780-8520, Japan; Research Laboratory of Hydrothermal Chemistry, Faculty of Science, Kochi, University, Akebono-cho 2-5-1, Kochi City, Kochi 780-8520, Japan.
³ Usa Marine Biological Institute, Kochi University, Inoshiri, Usa, Tosa, Kochi 781-1164, Japan.
⁴ Research Laboratory of Hydrothermal Chemistry, Faculty of Science, Kochi, University, Akebono-cho 2-5-1, Kochi City, Kochi 780-8520, Japan.
⁵ Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji 611-0011, Japan.

PMID: 27211652
DOI: 10.1016/j.foodchem.2016.04.121

Abstract

Microwave-assisted hydrothermal extraction was applied for production of sulfated polysaccharides from Ulva spp. and Monostroma latissimum. The maximum ulvan yields attained 40.4±3.2% (Ulva meridionalis) and 36.5±3.1% (Ulva ohnoi) within 4min of come-up time and 10min of extraction time at 160°C, respectively. The rhamnan sulfate yield from M. latissimum further attained 53.1±7.2% at 140°C. The sulfated polysaccharides were easily recovered from the extract by simple ethanol precipitation. In addition, molecular weights and viscosity of the extracted polysaccharides could be controlled by varying the extraction temperature. Dielectric measurement revealed that ionic conduction was the important parameter that affect the microwave susceptibility of algae-water mixture. The sulfated polysaccharides extracts are expected as potential feedstock for medical and food applications.

Keywords: Microwave-assisted hydrothermal extraction; Monostroma latissimum; Rhamnan sulfate; Ulva spp.; Ulvan.

MeSH terms

Chlorophyta / chemistry*
Deoxy Sugars / analysis*
Mannans / analysis*
Microwaves*
Polysaccharides / analysis*
Ulva / chemistry*
Viscosity

Substances

Deoxy Sugars
Mannans
Polysaccharides
ulvan
rhamnan