Antioxidant activity and potential photoprotective from amazon native flora extracts

Francislene J Martins; César A Caneschi; José L F Vieira; Wagner Barbosa; Nádia R B Raposo

doi:10.1016/j.jphotobiol.2016.05.012

Antioxidant activity and potential photoprotective from amazon native flora extracts

J Photochem Photobiol B. 2016 Aug:161:34-9. doi: 10.1016/j.jphotobiol.2016.05.012. Epub 2016 May 11.

Authors

Francislene J Martins¹, César A Caneschi¹, José L F Vieira², Wagner Barbosa², Nádia R B Raposo³

Affiliations

¹ Núcleo de Pesquisa e Inovação em Ciências da Saúde (NUPICS), Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, CEP:36036-330, Juiz de Fora, MG, Brazil.
² Faculdade de Ciências Farmacêuticas, Universidade Federal do Pará, Rua Augusto Corrêa, 01, CEP: 66075-740, Belém, PA, Brazil.
³ Núcleo de Pesquisa e Inovação em Ciências da Saúde (NUPICS), Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, CEP:36036-330, Juiz de Fora, MG, Brazil. Electronic address: nadiacritt@gmail.com.

PMID: 27208744
DOI: 10.1016/j.jphotobiol.2016.05.012

Abstract

Plant species are sources of active compounds that can fight and/or prevent damage caused by reactive oxygen species, which enables the development of natural products that can help to prevent premature aging caused by exposure to solar radiation. This study assessed the antioxidant and photoprotective activities of six dried extracts of plants from the Brazilian Amazon biome. Plant extracts were prepared in 70% (v/v) ethanol by dynamic maceration for 72h in the dark, and then filtered, concentrated and lyophilized. The extracts were subjected to a phytochemical screening. The antioxidant activity was measured using a 2,2-diphenyl-1-picrylhydrazyl assay and the photoprotection assay was performed using the diffuse transmittance technique. The data obtained from the antioxidant activity assay was evaluated by Student's t-test for independent samples, with the aid of Statistical Package for Social Sciences v.14.0 for Windows software. The flavonoids represent a special metabolites class present in all analyzed extracts. The antioxidant activity (μgmL(-1)) decreased in the following order: Aniba canelilla (1.80±0.16), Brosimum acutifolium (2.84±0.38), Dalbergia monetaria (5.46±0.17) or Caesalpinia pyramidalis (6.45±1.18), Arrabidaea chica (15.35±0.86), and Aspidosperma nitidum (99.14±2.3). Only D. monetaria showed a considerable sun protection factor allowing for labeling (6.0±0.3). The D. monetaria extract was considered the most promising sample because it had optimal antioxidant and photoprotective activities against solar radiation, considering the limit established by regulatory agencies. These extracts with antioxidant potential can be used in photoprotective formulations, providing synergistic photoprotective effect or elevating the adeed value of the product. Additionally, these formulations are attractive to a population who searchs for products made with natural ingredients.

Keywords: Amazon native flora; Antioxidant activity; Sun protection factor; Sunscreening agents.

MeSH terms

Antioxidants / chemistry*
Bignoniaceae / chemistry*
Bignoniaceae / metabolism
Brazil
Plant Extracts / chemistry*
Plant Leaves / chemistry
Plant Leaves / metabolism
Sun Protection Factor
Sunscreening Agents / chemistry*
Ultraviolet Rays

Substances

Antioxidants
Plant Extracts
Sunscreening Agents