Nitro-Triarylmethyl Radical as Dual Oxygen and Superoxide Probe

Benoit Driesschaert; Andrey A Bobko; Valery V Khramtsov; Jay L Zweier

doi:10.1007/s12013-016-0732-y

Nitro-Triarylmethyl Radical as Dual Oxygen and Superoxide Probe

Cell Biochem Biophys. 2017 Jun;75(2):241-246. doi: 10.1007/s12013-016-0732-y. Epub 2016 May 20.

Authors

Benoit Driesschaert^{1

2}, Andrey A Bobko^{3

4}, Valery V Khramtsov^{5

6}, Jay L Zweier⁷

Affiliations

¹ In Vivo Multifunctional Magnetic Resonance center, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, WV, 26506, USA. benoit.driesschaert@hsc.wvu.edu.
² Department of Biochemistry, West Virginia University School of Medicine, 1 Medical Center Drive, P.O. Box 9142, Morgantown, WV, 26506, USA. benoit.driesschaert@hsc.wvu.edu.
³ In Vivo Multifunctional Magnetic Resonance center, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, WV, 26506, USA.
⁴ Department of Biochemistry, West Virginia University School of Medicine, 1 Medical Center Drive, P.O. Box 9142, Morgantown, WV, 26506, USA.
⁵ In Vivo Multifunctional Magnetic Resonance center, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, WV, 26506, USA. valery.khramtsov@wvu.edu.
⁶ Department of Biochemistry, West Virginia University School of Medicine, 1 Medical Center Drive, P.O. Box 9142, Morgantown, WV, 26506, USA. valery.khramtsov@wvu.edu.
⁷ Davis Heart and Lung Research Institute, The Ohio State University/Wexner Medical Center, 460 West 12th Avenue, BRT 0390, Columbus, OH, 43210, USA. jay.zweier@osumc.edu.

Abstract

Superoxide radical is involved in numerous physiological and pathophysiological processes. Tetrathiatriarylmethyl (TAM) radicals are known to react with superoxide allowing measurement of superoxide production in biological media. We report the synthesis of a Nitro conjugated TAM radical showing a rate constant of 7 × 10⁵ M^-1 s^-1 which is two order of magnitude higher than other TAMs, allowing high sensitivity measurement of superoxide.

Keywords: EPR; Oxygen; ROS; Superoxide; Trityl.

MeSH terms

Electrochemical Techniques
Electron Spin Resonance Spectroscopy
Hydrogen-Ion Concentration
Molecular Probes / chemical synthesis
Molecular Probes / chemistry*
Oxygen / analysis*
Oxygen / chemistry
Solutions
Superoxides / analysis*
Superoxides / chemistry
Trityl Compounds / chemical synthesis
Trityl Compounds / chemistry*
Water / chemistry
Xanthine Oxidase / chemistry

Substances

Molecular Probes
Solutions
Trityl Compounds
Water
Superoxides
Xanthine Oxidase
Oxygen

Abstract

MeSH terms

Substances

Grants and funding