Characterization of two novel sialyl N-acetyllactosaminyl nucleotides separated from ovine colostrum

Glycoconj J. 2016 Oct;33(5):789-96. doi: 10.1007/s10719-016-9672-4. Epub 2016 May 20.

Abstract

The milk/colostrum of some mammalian species is known to contain sugar nucleotides including uridine diphosphate (UDP) oligosaccharides in addition to lactose and milk oligosaccharides, but the detailed structures of these UDP oligosaccharides have not so far been clarified. In this study we isolated two UDP-sialyl N-acetyllactosamines from ovine colostrum and characterized them using (1)H-NMR and MALDI-TOFMS spectroscopies. Their structures were found to be Neu5Gc(α2-3)Gal(β1-4)GlcNAcα1-UDP and Neu5Gc(α2-6)Gal(β1-4)GlcNAcα1-UDP.

Keywords: Milk oligosaccharide; Milk sugar nucleotide; Ovine colostrum; UDP oligosaccharide; UDP sialyl N-acetyllactosamine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Colostrum / chemistry*
  • Colostrum / metabolism
  • Female
  • Magnetic Resonance Spectroscopy
  • Sheep
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Uridine Diphosphate N-Acetylgalactosamine / analysis*
  • Uridine Diphosphate N-Acetylgalactosamine / metabolism

Substances

  • Uridine Diphosphate N-Acetylgalactosamine