One-step gene delivery into the cytoplasm in a fusion-dependent manner based on a new membrane fusogenic lipid

Hongzhang Deng; Xuefei Zhao; Dongxuan He; Weisheng Guo; Keni Yang; Anjie Dong; Xing-Jie Liang

doi:10.1039/c6cc01996j

One-step gene delivery into the cytoplasm in a fusion-dependent manner based on a new membrane fusogenic lipid

Chem Commun (Camb). 2016 May 31;52(46):7406-8. doi: 10.1039/c6cc01996j.

Authors

Hongzhang Deng¹, Xuefei Zhao², Dongxuan He¹, Weisheng Guo³, Keni Yang³, Anjie Dong⁴, Xing-Jie Liang³

Affiliations

¹ Department of Polymer Science and Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P. R. China. ajdong@tju.edu.cn and CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology, No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China.
² Department of Polymer Science and Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P. R. China. ajdong@tju.edu.cn.
³ CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology, No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China.
⁴ Department of Polymer Science and Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P. R. China. ajdong@tju.edu.cn and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China.

PMID: 27196702
DOI: 10.1039/c6cc01996j

Abstract

A new type of membrane fusogenic lipid was prepared to deliver DNA or siRNA into the cytoplasm directly in a fusion-dependent manner in order to bypass the cellular endocytosis to avoid the inefficient escape from the endosome and low transfection efficacy.

MeSH terms

Endocytosis
Gene Transfer Techniques*
Genetic Therapy / methods*
Humans
Membrane Fusion*
Membrane Lipids / chemistry*

Substances

Membrane Lipids