Performance of the HSV OligoGen kit for the diagnosis of herpes simplex virus type 1 and 2

Diagn Microbiol Infect Dis. 2016 Jul;85(3):315-317. doi: 10.1016/j.diagmicrobio.2016.04.019. Epub 2016 Apr 30.

Abstract

PCR methods are nowadays between the most rapid and sensitive methods for screening and diagnosing herpes simplex virus (HSV) type 1 and 2. The aim of this study was to analyze the reliability, accuracy, and usefulness of the new assay HSV OligoGen kit in comparison with the Roche LightCycler HSV ½ Qual Kit assay for the detection of HSV in clinical samples. For this analysis, a prospective study was designed for detection of HSV-1 and HSV-2 including 110 ulcer specimens, 48 urine, 48 endocervical, 43 cerebral spinal fluids, 4 urethral and 3 pharyngeal swabs that were sent from a regional STI clinic or an Intensive Clinical Unit, both in Seville, Spain. In comparison to the Roche LightCycler HSV ½ Qual Kit assay, sensitivity, specificity, positive and negative predicative values, and kappa value for HSV detection using the HSV OligoGen kit were 96.2%, 100%, 100%, 98.3%, and 0.97 for HSV-1, respectively. For HSV-2, the corresponding values were 98.3%, 100%, 100%, 99.5%, and 0.98, respectively. Statistical data obtained in this study confirms the usefulness and reliable results of this new assay.

Keywords: Diagnostic test; Genital herpes infection; HSV-1; HSV-2; Sexually transmitted infections.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adult
  • Female
  • Herpes Genitalis / diagnosis*
  • Herpes Simplex / diagnosis*
  • Herpesvirus 1, Human / genetics
  • Herpesvirus 1, Human / isolation & purification*
  • Herpesvirus 2, Human / genetics
  • Herpesvirus 2, Human / isolation & purification*
  • Humans
  • Male
  • Molecular Diagnostic Techniques / methods*
  • Predictive Value of Tests
  • Prospective Studies
  • Sensitivity and Specificity
  • Spain
  • Young Adult