High-sensitivity chemiluminescent immunoassay investigation and application for the detection of T-2 toxin and major metabolite HT-2 toxin

J Sci Food Agric. 2017 Feb;97(3):818-822. doi: 10.1002/jsfa.7801. Epub 2016 Jun 16.

Abstract

Background: T-2 toxin is a widely distributed mycotoxin in cereals. HT-2 toxin is the major metabolite, which is also a contaminant in cereals. T-2 toxin and HT-2 toxin have been identified as having carcinogenic, hepatotoxic, teratogenic and immunotoxic properties. To reduce the risk of contamination, a rapid, highly sensitive and inexpensive assay for the detection is required.

Results: In this study a high-sensitivity chemiluminescent enzyme-linked immunoassay (CL-ELISA) of T-2 toxin and HT-2 toxin was developed. With the help of the chemiluminescent substrate, this protocol showed a highly sensitive character with an IC50 as low as 33.28 ng mL-1 and 27.27 ng mL-1 for T-2 and HT-2, respectively. In addition, this method had no cross-reaction with other structurally related mycotoxins.

Conclusion: These results indicated that the developed CL-ELISA could be applied for the detection of T-2 toxin and HT-2 toxin in actual samples without complicated steps. © 2016 Society of Chemical Industry.

Keywords: CL-ELISA; HT-2 toxin; T-2 toxin; high-sensitivity; metabolite.

Publication types

  • Validation Study

MeSH terms

  • Antibodies, Monoclonal / metabolism
  • Carcinogens, Environmental / analysis*
  • Carcinogens, Environmental / metabolism
  • China
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Food Contamination*
  • Food Inspection / methods*
  • Limit of Detection
  • Luminescent Measurements
  • Methanol / chemistry
  • Oryza / chemistry*
  • Reproducibility of Results
  • Seeds / chemistry*
  • Solubility
  • Solvents / chemistry
  • T-2 Toxin / analogs & derivatives*
  • T-2 Toxin / analysis*
  • T-2 Toxin / metabolism

Substances

  • Antibodies, Monoclonal
  • Carcinogens, Environmental
  • Solvents
  • T-2 Toxin
  • HT-2 toxin
  • Methanol