Effect of starch on the mechanical and in vitro properties of collagen-hydroxyapatite sponges for applications in dentistry

Ana B Castro-Ceseña; Tanya A Camacho-Villegas; Pavel H Lugo-Fabres; Ekaterina E Novitskaya; Joanna McKittrick; Alexei Licea-Navarro

doi:10.1016/j.carbpol.2016.04.056

Effect of starch on the mechanical and in vitro properties of collagen-hydroxyapatite sponges for applications in dentistry

Carbohydr Polym. 2016 Sep 5:148:78-85. doi: 10.1016/j.carbpol.2016.04.056. Epub 2016 Apr 14.

Authors

Ana B Castro-Ceseña¹, Tanya A Camacho-Villegas², Pavel H Lugo-Fabres³, Ekaterina E Novitskaya⁴, Joanna McKittrick⁵, Alexei Licea-Navarro⁶

Affiliations

¹ Materials Science and Engineering Program, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: acastro@cideteq.mx.
² Biomedical Innovation Department, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana No. 3918, Zona Playitas, C.P. 22860, Ensenada, Baja California, México. Electronic address: tcamacho@ciatej.mx.
³ Biomedical Innovation Department, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana No. 3918, Zona Playitas, C.P. 22860, Ensenada, Baja California, México. Electronic address: plugo@cicese.edu.mx.
⁴ Department of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: eevdokim@ucsd.edu.
⁵ Materials Science and Engineering Program, University of California, San Diego, La Jolla, CA 92093, USA; Department of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: jmckittrick@eng.ucsd.edu.
⁶ Biomedical Innovation Department, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana No. 3918, Zona Playitas, C.P. 22860, Ensenada, Baja California, México. Electronic address: alicea@cicese.mx.

PMID: 27185118
DOI: 10.1016/j.carbpol.2016.04.056

Abstract

This study sought to improve the mechanical and blood-absorbing properties of collagen sponges, while keeping them compressible, by incorporating blended hydroxyapatite (HA)-starch. Results were compared with CollaPlug(®) (pure collagen). The elastic modulus increased from 1.5±0.2kPa for CollaPlug(®) to 49±8kPa for sponges with composition 1:4:10 (collagen:HA:starch, by weight). The modified microstructure and surface area provided by the starch granules on the sponges improved cell viability. Sponges with composition 1:4:10 maintained their blood-clotting capability with almost no change from 5 to 15min after contact with blood, while CollaPlug(®) diminished to about half its capacity to absorb blood and form clots. Incorporation of HA-starch into the sponges with composition of 1:4:10, increased the elastic modulus of the collagen-HA sponges, making them more structurally robust. The viability of cells and the blood-clotting capability increased with starch incorporation.

Keywords: Blood-clotting; CollaPlug(®); Collagen; Dental filler; Sponge; Starch.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dentistry*
Durapatite / chemistry*
Elastic Modulus / drug effects
Humans
Starch / chemistry*
Starch / pharmacology

Substances

Starch
Durapatite