Abstract
The fluorescence of BODIPY and click-BODIPY dyes was found to substantially increase in the presence of bovine serum albumin (BSA). BSA acted as a solubilizer for dye aggregates, in addition to being a conventional binding scaffold for the click-BODIPY dyes, indicating that disaggregation of fluorophores should be considered when evaluating dye-protein interactions.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Boron Compounds / chemistry
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Boron Compounds / metabolism*
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Cattle
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Click Chemistry
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism
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Protein Binding
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Serum Albumin, Bovine / chemistry
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Serum Albumin, Bovine / metabolism*
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Spectrometry, Fluorescence
Substances
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4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
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Boron Compounds
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Fluorescent Dyes
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Serum Albumin, Bovine