Characterisation of fucoidan extracted from Malaysian Sargassum binderi

Seng Joe Lim; Wan Mustapha Wan Aida; Mohamad Yusof Maskat; Jalifah Latip; Khairiah Haji Badri; Osman Hassan; Bohari M Yamin

doi:10.1016/j.foodchem.2016.04.058

Characterisation of fucoidan extracted from Malaysian Sargassum binderi

Food Chem. 2016 Oct 15:209:267-73. doi: 10.1016/j.foodchem.2016.04.058. Epub 2016 Apr 18.

Authors

Seng Joe Lim¹, Wan Mustapha Wan Aida², Mohamad Yusof Maskat³, Jalifah Latip⁴, Khairiah Haji Badri⁵, Osman Hassan⁶, Bohari M Yamin⁷

Affiliations

¹ School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: joe@ukm.edu.my.
² School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: wanaidawm@ukm.edu.my.
³ School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: maskatmy@gmail.com.
⁴ School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: jalifah@ukm.edu.my.
⁵ School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: kaybadri@ukm.edu.my.
⁶ School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: drosmanhassan@gmail.com.
⁷ School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia. Electronic address: bohari@ukm.edu.my.

PMID: 27173562
DOI: 10.1016/j.foodchem.2016.04.058

Abstract

Fucoidan is a sulphated polysaccharide that consists mainly of fucose, normally found in brown seaweeds. In this study, fucoidan was extracted from Sargassum binderi (Fsar) from Malaysia and subsequently characterised. The chemical characteristics of Fsar were found to be different than those of commercial food grade fucoidan (Fysk) and those of previously studied fucoidans. NMR analysis proposed that the main structure of Fsar is →3)fuc-2-OSO3(-)(1→3)fuc(1→. The molecular weight (47.87kDa) and degree of sulphation (0.20) of Fsar were higher than those of Fysk, at 27.98kDa and 0.15, respectively. However, Fsar's polydispersity index (1.12) and fucose content (34.50%) were lower than those of Fysk, at 1.88 and 43.30%, respectively. Both Fsar and Fysk showed similar thermo-gravimetric properties with four mass losses, amorphous in nature and negative optical rotations. Results show that Fsar has fundamental characteristics of fucoidan with different structural conformation i.e. variation in glycosidic linkages and sulphate group orientation.

Keywords: Characterisation; Fucoidan; Sargassum binderi; Structure analysis.

MeSH terms

Fucose / isolation & purification*
Magnetic Resonance Spectroscopy
Malaysia
Molecular Weight
Plant Extracts / chemistry*
Polysaccharides / isolation & purification*
Sargassum / chemistry*
Sargassum / growth & development
Seaweed / chemistry*
Seaweed / growth & development

Substances

Plant Extracts
Polysaccharides
Fucose
fucoidan