Abstract
A novel molecule, TatBim-Alexa, consisting of the HIV1 Tat cell-penetrating peptide, the Bim apoptosis-inducing peptide, and Alexa Fluor 546 was synthesized for photoinducion of apoptosis. The Alexa Fluor 546 was used as a photosensitizer and covalently attached at the C-terminus of TatBim peptide by the thiol-maleimide reaction. Photo-dependent cytosolic internalization of TatBim-Alexa and photo-dependent apoptosis using TatBim-Alexa were demonstrated in several kinds of mammalian cells including human cancer cell lines.
Keywords:
Apoptosis; Bim; Cell-penetrating peptide; Photosensitizer.
Copyright © 2016 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents / chemical synthesis
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / pharmacology*
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Apoptosis / drug effects*
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CHO Cells
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Cricetulus
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Dose-Response Relationship, Drug
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Drug Screening Assays, Antitumor
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / pharmacology*
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HeLa Cells
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Humans
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Molecular Structure
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Peptides / chemistry
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Peptides / pharmacology*
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Photochemical Processes
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Photosensitizing Agents / chemical synthesis
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Photosensitizing Agents / chemistry
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Photosensitizing Agents / pharmacology*
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Quinolinium Compounds / chemical synthesis
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Quinolinium Compounds / chemistry
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Quinolinium Compounds / pharmacology*
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Structure-Activity Relationship
Substances
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Alexa fluor 546
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Antineoplastic Agents
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Fluorescent Dyes
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Peptides
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Photosensitizing Agents
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Quinolinium Compounds