Chloroplast simple sequence repeat (cpSSR) markers previously reported were used to analyze chloroplast diversity of Chinese grapes. Nine cpSSR primer pairs from the chloroplast genomes of citrus and tobacco (Nicotiana tabacum L.) were found useful for analyzing the grapes chloroplast genome (cpDNA). Selected cpSSR primer pairs showed polymorphism among 64 varieties, with polymorphism information content (PIC) values ranging from 0.522 to 0.659, with an average of 0.590. Results depicted that a total number of 43 alleles were detected on nine loci with an average of 4.77 alleles per locus with highest number of six alleles for marker ARCP2 and CCMP10. Gene diversity explained by these cpSSR makers ranged from 4 to 68% with an average of 63.66% per marker. The genetic relationship suggested by UPGMA based dendrogram divided the genotypes into three main clusters and four subgroups, results showed that 2B is the biggest group. Overall Hong Ma Ge and Da LI Mei Gui Xiung 98% similar, while 64-1-19 was the most diverse as compared to rest of genotypes.
Keywords: Chloroplast; genetic diversity; phylogenetic associations; vitis vinifera.